姓名：张连文

职称：副教授

学科方向：微生物与生化药学

研究方向： 糖生物学

通讯地址：南开大学药学院 天津海河教育园同砚路38号，300353

电子邮件：lianwen@nankai.edu.cn

教师简介：

1997年本科毕业于山东师范大学化学系，；2000年硕士毕业于中科院上海应用物理研究所；2007年博士毕业于清华大学生物系。2007.7进入南开大学药学院工作，从事糖生物学（主要为蛋白O-GlcNAc修饰）研究。先后围绕糖基转移酶检测方法与底物特异性、O-GlcNAc修饰与泛素化修饰关系、肿瘤细胞中O-GlcNAc修饰变化进行了深入研究。目前科研内容包括：（1）OGT亚型底物特异性研究；（2） O-GlcNAc修饰对重要功能蛋白的影响；（3）O-GlcNAc修饰相关酶抑制剂筛选；（4）O-GlcNAc修饰与代谢疾病。

代表性论文：

1.  Zhang LW*, Ren FF, Li J,et al. A modified coupled-enzyme method for O-linked GlcNAc transferase activity assay. Biological procedures online, 2009,11(1): 171-183

2.  Rui Shen, Shuai Wang, Junyang Xian, Xiaofeng Ma, Jing Li, Lianwen Zhang* and Peng Wang, An easy colorimetric assay for several glycosyltransferase, Biochemistry-Moscow, 2010, 75(7): 944-950.

3. Guangxiang Ren, Jingli Hou, Qinghong Fang, Hong Sun, Peng George Wang and Lianwen Zhang* Synthesis of flavonol 3-O-glycoside by UGT78D1, Glycoconjugate Journal, 2012, 29 (5):425-432

4.  Xiaofeng Ma, Pi Liu, Hui Yan, Hong Sun, Xiaoyan Liu, Feng Zhou, Lei Li, Yi Chen, Musleh M. Muthana, Xi Chen, Peng george Wang* and Lianwen Zhang*. Substrate specificity provides insights into the molecular basis of O-GlcNAc transferase (OGT) – sugar donor recognition. PLoS ONE. 2013, 8 (5): e63452.

5. Zhou, F., H. Yan, L. Li, X. Y. Liu, Y. Q. Wang, P. Wang* and L. W. Zhang* FBXW10 is negatively regulated in transcription and expression level by protein O-GlcNAcylation. Biochem Biophys Res Commun.  2013,438(2): 427-432.

6.  Xiaoyan Liu, Ling Li, Yuqiu Wang, Hui Yan, Xiaofeng Ma, Peng George Wang, Lianwen Zhang*, A peptide panel investigation reveals the acceptor specificity of O-GlcNAc Transferase, FASEB J.  2014, 28(8) : 3362-3372.

7.  Han, X., L. Qian, L. Zhang* and X. Liu* Structural and biochemical insights into nucleotide-rhamnose synthase/epimerase-reductase from Arabidopsis thaliana. Biochim Biophys Acta 2015, 1854(10 Pt A): 1476-1486.

8.  Jiang, K., Y. Gao, W. Hou, F. Tian, W. Ying, L. Li, B. Bai, G. Hou*, P. G. Wang* and L. Zhang* Proteomic analysis of O-GlcNAcylated proteins in invasive ductal breast carcinomas with and without lymph node metastasis. Amino Acids 2016, 48(2): 365-374.

9.Jiang, K., B. Bai, Y. Ta, T. Zhang, Z. Xiao, P. G. Wang* and L. Zhang* O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway. Biochem Biophys Res Commun. 2016, 471(4):539-44

10. Wu, Zhigang; Jiang, Kuan; Zhu, Hailiang; Ma, Cheng; Yu, Zaikuan; Li, Lei; Guan, Wanyi; Liu, Yunpeng; Zhu, He; Chen, Yanyi; Li, Shanshan; LI, Jing; Cheng, Jiansong; Zhang, Lianwen* and Wang, Peng*,  Site-directed glycosylation of peptide/protein with homogeneous O-linked eukaryotic N-glycans, Bioconjugate Chemistry, 2016, 27 (9), pp 1972–1975.

11. Kuan Jiang, Arya Aloor, Jiangyao Qu, Cong Xiao, Zhigang Wu, Cheng Ma, Lianwen Zhang*, Peng George Wang *, Rapid and sensitive MALDI MS analysis of oligosaccharide by using 2-hydrazinopyrimidine as a derivative reagent and co-matrix, Anal Bioanal Chem, (DOI: 10.1007/s00216-016-9690-x), Accepted.

申请专利：

张连文，马晓峰等。 一种半乳糖基转移酶修饰黄酮类糖苷化合物的方法，（公开号：CN 200910068855.7）