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程剑松

姓名:程剑松

职称:副研究员

学科方向: 微生物制药

研究方向:糖生物学,糖化学生物学

通讯地址:南开大学药物化学生物学国家重点实验室综合实验楼D221

电子邮件:jiansongcheng@nankai.edu.cn

教师简介

1996-2000,南开大学生命科学学院(生化与分子生物学,本科)

2001-2006,南开大学(微生物学,博士)

2006.12-2009.12, University of California, Davis(化学系,博士后)。

研究领域:糖基转移酶的催化机制及其在碳水化合物生化合成(Chemoenzymatic synthesis)方面的应用以及N-蛋白的糖基化修饰。

代表性论文:

1. Zhu H, Li X, Qu J, Xiao C, Jiang K, Gashash E, Liu D, Song J, Cheng J, Ma C,Wang PG. Diethylaminoethyl Sepharose (DEAE-Sepharose) microcolumn for enrichment of glycopeptides. Anal Bioanal Chem. 2016 Sep 27. PMID: 27679458.

2. Song Q, Zhang X, Li N, Shen J, Cheng J. A propeptide-independent protease from Tannerella sp.6_1_58FAA_CT1 displays trypsin-like specificity. J Basic Microbiol. 2016,56,1-7. doi: 10.1002/jobm.201600486. PMID:27714828.

3. Wu Z, Jiang K, Zhu H, Ma C, Yu Z, Li L, Guan W, Liu Y, Zhu H, Chen Y, Li S, Li J, Cheng J, Zhang L, Wang PG. Site-Directed Glycosylation of Peptide/Protein with Homogeneous O-Linked Eukaryotic N-Glycans. Bioconjug Chem. 2016 Sep 21;27(9):1972-5. doi: 10.1021/ acs.bioconjchem.6b00385. PMID: 27529638.

4. Wang Y, Jiang K, Ma H, Zeng W, Wang PG, Yao N, Han W, Cheng J, Wang W. Enzymatic production of HMO mimics by the sialylation of galacto-oligosaccharides. Food Chem. 2015 Aug 15;181:51-6. doi: 10.1016/j.foodchem.2015.02.064. PMID: 25794720.

5. Wang W, Ma T, Zhang B, Yao N, Li M, Cui L, Li G, Ma Z, Cheng J. A novel mechanism of protein thermostability: a unique N-terminal domain confers heat resistance to Fe/Mn-SODs. Sci Rep. 2014 Dec 2;4:7284. doi: 10.1038/srep07284. PMID: 25445927.

6. Meng X, Yao W, Cheng J, Zhang X, Jin L, Yu H, Chen X, Wang F, Cao H. Regioselective chemoenzymatic synthesis of ganglioside disialyl tetrasaccharide epitopes. J Am Chem Soc. 2014 Apr 9;136(14):5205-8. doi: 10.1021/ja5000609. PMID: 24649890

7. Wang L, Han W, Xie C, Hou J, Fang Q, Gu J, Wang PG, Cheng J. Comparing the acceptor promiscuity of a Rosa hybrida glucosyltransferase RhGT1 and an engineered microbial glucosyltransferase OleD(PSA) toward a small flavonoid library. Carbohydr Res. 2013 Mar 7;368:73-7. doi: 10.1016/j.carres.2012.12.012. PMID: 23337250.

8. Han W, Cai L, Wu B, Li L, Xiao Z, Cheng J, Wang PG. The wciN gene encodes an α1,3-galactosyltransferase involved in the biosynthesis of the capsule repeating unit of Streptococcus pneumoniae serotype 6B. Biochemistry. 2012 Jul 24;51(29):5804-10. doi: 10.1021/bi300640b. PMID: 22742596

9. Xie C, Han W, Wang PG, Cheng J. Probing the role of highly conserved residues forming the acceptor binding pocket of the promiscuous glycosyltransferase MGT in defining the specificity towards a panel of flavonoids. Biochemistry (Mosc). 2013:78(5):536-41.

10. Chen X, Wang Y, Ma Z, Li N, Han W, Zhang Q, Cai Y, Cheng J. Asp141 and the Hydrogen-Bond Chain Asp141-Asn109-Asp33 Are Respectively Essential for GT80 Sialyltransferase Activity and Structural Stability.

Biochemistry (Mosc). 2015;80(8):1073-9. doi: 10.1134/ S0006297915080131.PMID:26547076.

11. Padler-Karavani V, Song X, Yu H, Hurtado-Ziola N, Huang S, Muthana S, Chokhawala HA, Cheng J, Verhagen A, Langereis MA, Kleene R, Schachner M, de Groot RJ, Lasanajak Y, Matsuda H, Schwab R, Chen X, Smith DF, Cummings RD, Varki A. Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays. J Biol Chem. 2012 Jun 29;287(27):22593-608. doi: 10.1074/jbc.M112.359323. PMID: 22549775.

12. Chen GY, Chen X, King S, Cavassani KA, Cheng J, Zheng X, Cao H, Yu H, Qu J,Fang D, Wu W, Bai XF, Liu JQ, Woodiga SA, Chen C, Sun L, Hogaboam CM, Kunkel SL,Zheng P, Liu Y. Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction. Nat Biotechnol. 2011 May;29(5):428-35. doi: 10.1038/nbt.1846. PMID: 21478876.

13. Zhang L, Lau K, Cheng J, Yu H, Li Y, Sugiarto G, Huang S, Ding L, Thon V,Wang PG, Chen X. Helicobacter hepaticus Hh0072 gene encodes a novel

alpha1-3-fucosyltransferase belonging to CAZy GT11 family. Glycobiology. 2010 Sep;20(9):1077-88. doi: 10.1093/glycob/cwq068. PMID: 20466652.

14. Cao H, Li Y, Lau K, Muthana S, Yu H, Cheng J, Chokhawala HA, Sugiarto G,Zhang L, Chen X. Sialidase substrate specificity studies using chemoenzymatically synthesized sialosides containing C5-modified sialic acids. Org Biomol Chem. 2009 Dec 21;7(24):5137-45. doi: 10.1039/b916305k. PMID: 20024109.

15. Yu H, Cheng J, Ding L, Khedri Z, Chen Y, Chin S, Lau K, Tiwari VK, Chen X. Chemoenzymatic synthesis of GD3 oligosaccharides and other disialyl glycans containing natural and non-natural sialic acids. J Am Chem Soc. 2009 Dec 30;131(51):18467-77. doi: 10.1021/ja907750r. PMID: 19947630

16. Cheng J, Huang S, Yu H, Li Y, Lau K, Chen X. Trans-sialidase activity of Photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides. Glycobiology. 2010 Feb;20(2):260-8. doi:

10.1093/glycob/cwp172. PMID: 19880425.

17. Cao H, Muthana S, Li Y, Cheng J, Chen X. Parallel chemoenzymatic synthesis of sialosides containing a C5-diversified sialic acid. Bioorg Med Chem Lett. 2009;19(20):5869-71.doi: 10.1016/j.bmcl.2009.08.078. PMID: 19740656.

18. Muthana S, Yu H, Cao H, Cheng J, Chen X. Chemoenzymatic synthesis of a new class of macrocyclic oligosaccharides. J Org Chem. 2009 Apr 17;74(8):2928-36. doi: 10.1021/jo8027856. PMID: 19296596.

19. Feng L, Reeves PR, Lan R, Ren Y, Gao C, Zhou Z, Ren Y, Cheng J, Wang W, Wang J, Qian W, Li D, Wang L. A recalibrated molecular clock and independent origins for the cholera pandemic clones. PLoS One. 2008;3(12):e4053. doi: 10.1371/journal.pone.0004053. PMID: 19115014.

20. Chokhawala HA, Huang S, Lau K, Yu H, Cheng J, Thon V, Hurtado-Ziola N,Guerrero JA, Varki A, Chen X. Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides. ACS Chem Biol. 2008 Sep 19;3(9):567-76. doi: 10.1021/cb800127n. PMID: 18729452.

21. Wang Z, Gilbert M, Eguchi H, Yu H, Cheng J, Muthana S, Zhou L, Wang PG, Chen X, Huang X. Chemoenzymatic Syntheses of Tumor-Associated Carbohydrate Antigen Globo-H and Stage-Specific Embryonic Antigen 4. Adv Synth Catal. 2008;350(11-12):1717-1728. PMID: 20305750.

22. Cheng J, Yu H, Lau K, Huang S, Chokhawala HA, Li Y, Tiwari VK, Chen X. Multifunctionality of Campylobacter jejuni sialyltransferase CstII:

characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. Glycobiology. 2008;18(9):686-97.doi: 10.1093/glycob/cwn047. PMID: 18509108.

23. Feng L, Wang W, Cheng J, Ren Y, Zhao G, Gao C, Tang Y, Liu X, Han W, Peng X, Liu R, Wang L. Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. Proc Natl Acad Sci U S A. 2007;104(13):5602-7. PMID: 17372208.


 



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