师资队伍教授
郗日沫

姓名:郗日沫

职称:教授

学科方向:生物抗体技术;伴随病理免疫诊断;免疫分析检测;电化学分析

招生方向:药物化学

课题组成员

副教授:孟萌博士

讲师:尹永梅博士

通讯地址天津市南开大学津南校区药学院237

传真:022-22507760

电子邮件:xirimo@nankai.edu.cn

教师简介:

郗日沫,男,日本文部省冈崎国立共同研究机构分子科学研究所, 日本综合研究大学院大学理学博士。1962年5月13日出生于内蒙古。1980年考入南开大学化学系,1987年毕业获化学硕士学位。1987年至1990年在北京大学化学系攻读博士学位。 1995年4月在日本文部省冈崎国立共同研究机构分子科学研究所, 日本综合研究大学院大学获理学博士学位。1995年至1997年在美国芝加哥大学化学系从事博士后研究。1997年至1998年在美国威斯康星大学麦迪逊分校任博士后研究员。从1998-2003起,在美国生物技术公司从事生物技术免疫诊断试剂的研究,开发和市场化。2003年3月,成为山东大学化学与化工学院教授,组建了食品中药物残留免疫快速检测实验室,专门从事食品残留药物的免疫原的合成和相应免疫检测方法的研究。2009年1月,调到南开大学药学院担任药物分析教授,从事血药浓度检测及电化学免疫分析。

教育经历

Postdoc. (1997-1998): Chemistry, The Universiyt of Wisconsin-Madison, USA.

Postdoc. (1995-1997): Chemistry, The University of Chicago, Chicago, USA.

Ph.D.     (1992-1995): Chemistry, Institute for Molecular Science, Okazaki, Japan.

M.S.      (1984-1987): Chemistry, Nankai University, Tianjin, China.

B.S.       (1980-1984): Chemistry, Nankai University, Tianjin, China.

研究领域和兴趣

1. 病理抗体制备和IHC检测技术研发.

2. 肿瘤伴随诊断技术的研究开发.

3. 小分子抗原的合成和多克隆/单克隆抗体制备

4. 生物免疫技术药物残留检测方法的研究和开发

5. 血药浓度监测关键抗体试剂的联合研发

6. 电化学免疫分析法的建立及应用

主持的科研项目

1. 国家科技部重大国际科技合作项目 (主持人),2015DFR40460,血药浓度监测关键抗体试剂的联合研发,2015.4-2018.3.

2. 国家科技部863计划 (主持人), 2014AA022303, 儿童营养素抗体检测技术体系的建立, 2014.12-2016.12.

3. 国家科技部863计划 (主持人), 2007AA10z435, 重要禁用兽药快速免疫检测技术及产品, 2007.8-2010.10.

4. 国家科技部重大863计划 (子课题主持人), 2007AA06A407,重大环境污染事件特征污染物实验室检测技术系统,2007.11-2010.11

5. 国家自然科学基金-面上项目(主持人),81173017、新药筛选关键技术甲基化转移酶活性的免疫检测体系的研究、2012/1-2015/12.

6. 国家自然科学基金-面上项目(主持人), 20685048, 硝极呋喃类药物抗体的制备和酶联免疫分析法的建立, 2007.1-2009.12.

7. 天津市中高端医疗器械重大专项(主持人), 糖化血红蛋白即时检测产品的开发研究, 2016/12-2019/12.

8. 天津市科技支撑计划重点项目(主持人),11ZCGHHZ01200、喹诺酮药物的多残留检测及抗体亲和力构效关系的分子模拟研究、2011/10-2013/09

9. 天津市科技计划项目(主持人),13JCZDJC29700、食品中的邻苯二甲酸酯的快速荧光偏振免疫分析方法研究、2013/04-2016/03;

代表性论文:

1. Xiaogang Li, Meng Meng*, Lei Zheng, Rimo Xi, Chemiluminescence Immunoassay for S-Adenosylhomocysteine Detection and Its Application in DNA Methyltransferase Activity Evaluation and Inhibitors Screening, Anal. Chem., 2016, 88 (17), pp 8556–8561

2. Kun Xu, Hao Long, Rongge Xing, Meng Meng*; Rimo Xi*, A sensitive chemiluminescent immunoassay to detect Chromotrope FB (Chr FB) in foods, Talanta, (2016) http://dx.doi.org/10.1016/j.talanta.2016.09.063

3. L.S. Zhang, Z.R. Song, Y.Q. Dong, Y.F. Wang, X.G. Li, H. Long, K. Xu, C. Deng, M. Meng, Y.M. Yin, R.M. Xi, Enantiomeric separation of 1,4-dihydropyridines by liquid-phase microextraction with supercritical fluid chromatography, J. Supercrit. Fluids 107 (2016) 129-136.

4. Lisha Zhang, Hao Long, Xiaogang Li, Kun Xu, Meng Meng, Yongmei Yin, Rimo Xi. Production of a sensitive antibody against sirolimus for chemiluminescence immunoassay potential in its therapeutic drug monitoring. Analytical Methods, 2016, 8, 6298-6304

5. Jing Wang, Lei Zheng, Yaqing Dong, Zhaorui Song, Yufen Wang, Meng Meng, Linlin Ren,  Sergei A. Eremin, Chuan Deng, Yongmei Yin, Rimo Xi*. Establishment of Enhanced Chemiluminescent ImmunoassayFormats for Stanozolol Detection in animal-derived foodstuffsand Other Matrices, Food Anal. Methods (2016) 9:1284–1292.

6. Zhaorui Song, Yufen Wang, Yaqing Dong, Kun Xu, Hao Long, Chuan Deng, Yongmei Yin, Sergei A. Eremin, Meng Meng* and Rimo Xi*, A validated chemiluminescence immunoassay for methotrexate (MTX) and its application, Anal. Methods, 2016, 8, 162-170.

7. Lei Zheng#; Jing Wang#; Jie Zhang#; Zhaorui Song; Yaqing Dong; Yufen Wang; Zhongsheng Tong; Chuan Deng; Yongmei Yin; Meng Meng*; Rimo Xi*,Preparation of polyclonal antibodies for nateglinide (NTG) anddevelopment of a sensitive chemiluminescent immunoassay to detectNTG in tablets and serum, Talanta, 146 (2016) 483 – 489.

8. Lei Zheng#, Jing Wang#, Yufen Wang, Zhaorui Song, Yaqing Dong, Yongmei Yin & Sergei A. Eremin, Meng Meng*, Rimo Xi*, Anal Bioanal Chem (2015) 407:1973–1980.

9. Yaqing Dong, Jie Zhang, Yue Xing, Zhaorui Song, Yufen Wang, Meng Meng, Chuan Deng, Zhongsheng Tong, Yongmei Yin, and Rimo Xi*, Quantification of Ponceau 4R in Foods by Indirect Competitive Enzyme-Linked Immunosorbent Assay (icELISA), J. Agric. Food Chem., 2015,63 (28): 6338-6345.

10. Linlin Ren, Meng Meng, Peng Wang, Zhihuan Xu, Sergei A . Eremin, Junhong Zhao, Yongmei Yin*, Rimo Xi*, Determination of sodium benzoate in food products by fluorescence polarization immunoassay. Talanta, 121 (2014) 136-143.

11. Peng Wang, Yongmei Yin, Sergei A. Eremin, Victor B. Rybakov, Taichang Zhang, Zhihuan Xu, Linlin Ren, Xiaodan He, Meng Meng*, Rimo Xi*, An Indirect Competitive Immunoassay for Detection of Vitamin B2 in Foods and Pharmaceuticals, J. Agric. Food Chem., 2013, 61, 7048?7054.

12. Bo Zhang, Daolin Du, Meng Meng, Sergei A. Eremin, Victor B. Rybakov, Junhong Zhao, Yongmei Yin*, Rimo Xi*. Determination of Amaranth in Beverage by Indirect Competitive Enzyme-linked Immunosorbent Assay (ELISA) Based on Anti-amaranth Monoclonal Antibody. Food Anal. Methods, 2013, DOI 10.1007/s12161-013-9779-1.

13. Huyin Xue, Yue Xing, Yongmei Yin, Taichang Zhang, Bo Zhang, Yu Zhang, Pei Song, Xi Tian,Yinghui Xu, Peng Wang, Meng Meng* and Rimo Xi*, Application of an enzyme immunoassay for the quantitative determination of azo dye (Orange II) in food products, Food Additives & Contaminants: Part A, 2012, 29:1840–1848.

14. Xiao Lu, Hong Zheng, Xiuqing Li, Xuexia Yuan, Hui Li, Ligang Deng, Hong Zhang, Wenzheng Wang, Guosheng Yang*, Meng Meng, Rimo Xi*, Hassan Y. Aboul-Enein*, Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay, Food Chem, 2012, 130: 1061-1065.

15. Yue Xing, Meng Meng, Huyin Xue, Taichang Zhang, Yongmei Yin*, Rimo Xi*. Development of a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for detection of Sunset Yellow FCF in food samples. Talanta, 2012, 99: 125–131.

16. Jing Xu, Weiwei Yin, Yuanyang Zhang, Jian Yi, Meng Meng*, Yabin Wang, Huyin Xue, Taichang Zhang, Rimo Xi*, Establishment of magnetic beads-based enzyme immunoassay for detection of chloramphenicol in milk. Food Chemistry, 2012, 134: 2526–2531.

17. Xiaoling Fu, Meng Meng*, Yu Zhang, Yongmei Yin, Xiangsheng Zhang*, Rimo Xi*, Chemiluminescence enzyme immunoassay using magnetic nanoparticles for detection of neuron specific enolase in human serum, Analytica Chimica Acta, 2012, Doi:10.1016/j.aca.2012.02.007.

18. Taichang Zhang, Huyin Xue, Bo Zhang, Yu Zhang, Pei Song, Xi Tian, Yue Xing, PengWang, Meng Meng* and Rimo Xi*, Determination of folic acid in milk, milk powder and energy drinks by an indirect immunoassay, J Sci. Food Agric., 2012, 92, DOI:10.1002/jsfa.5625.

19. Jian Yi, Meng Meng, Zhongqiu Liu, Jinfang Zhi, Yuanyang Zhang, Jing Xu, Yabin Wang, Jinting Liu*, Rimo Xi*, Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine, J Zhejiang Univ-Sci B, 2012, 13(2):118-125.

20. Yabin Wang, Fangyang He, Yuping Wan, Meng Meng*, Jing Xu, Yuanyang Zhang, Jian Yi, Caiwei Feng, Shanliang Wang, Rimo Xi*. An indirect competitive enzyme-linked immuno-sorbent assay (ELISA) to detect nitroimidazoles in food products, Food Additives & Contaminants: Part A, 2011, 28: 619-626.

21. Meng Meng, Rimo Xi*. Review: Current development in analyzing drug residue in foods and food products. Analytical Letters, 2011, 44: 2543-2558.

22. Weihua Li, Meng Meng, Huyin Xue, Wei Liu, Weiwei Yin, Jing Xu, Fangyang He, Yuping Wan, Caiwei Feng, Shanliang Wang, Xiao Lu, Jinting Liu*, Rimo Xi*. Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA for detection of diethylstilbestrol in animal tissue, urine and bile. Chinese Science Bulletin, 2011, 56(8): 749-754.

23. Zhaozhen Cao, Shengxin Lu, Jinting Liu, Jinhua Zhan, Meng Meng*, Rimo Xi*. Preparation of anti-Lomefloxacin Antibody and Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Lomefloxacin Residue in Milk. Analytical Letters, 2011, 44 (6), 1100-1113.

24. Zhaozhen Cao, Meng Meng*, Shengxin Lu, Rimo Xi*. Development of an indirect chemiluminescent competitive ELISA to detect danofloxacin residues in milk. Analytical Letters, 2011, 44 (6), 1077-1084.

25.Yuanyang Zhang, Fangyang He, Yuping Wan, Meng Meng*, Jing Xu, Jian Yi, Yabin Wang, Caiwei Feng, Shanliang Wang, Rimo Xi*. Generation of anti-trenbolone monoclonal antibody and establishment of an indirect competitive enzyme-linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine. Talanta, 2011, 83, 732-737.

26. Jing Xu, Yuanyang Zhang, Jian Yi, Meng Meng*, Rimo Xi*. Preparation of anti-Sudan Red monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of Sudan Red in Chili Jam and Chili Oil, Analyst, 2010,135(10), 2566-2572.

27. Weiwei Yin, Jinting Liu, Weihua Li, Wei Liu, Meng, Meng*, Yuping Wan, Caiwei Feng, Shanliang Wang, Xiao Lu, Rimo Xi*. Preparation of monoclonal antibody for melamine and development of an indirect competitive ELISA for melamine detection in raw milk, milk Powder and animal Feeds, J. Agric. Food Chem., 2010, 58, 8152-8157.

28. Wei Liu, Weihua Li, Weiwei Yin, Meng Meng*, Yuping Wan, Caiwei Feng, Shanliang Wang and Rimo Xi*. Preparation of monoclonal antibody and development of an indirect competitive ELISA for the detection of chlorpromazine residue in chicken and swine liver, J Sci Food Agr, 2010, 90, 1789-1795.

29. Yongchao Lai, Weiwei Yin, Jinting Liu, Rimo Xi, Jinhua Zhan, One-Pot Green Synthesis and Bioapplication of L-Arginine-Capped Superparamagnetic Fe3O4 Nanoparticles, Nanoscale Res. Lett., 2010, 5, 302–307

30. Kai Ding, Cuihua Zhao, Zhaozhen Cao, Zhongqiu Liu, Jinting Liu, Jinhua Zhan, Chen Ma, and Rimo Xi*. Chemiluminescent Detection of Gatifloxacin Residue in Milk. Analytical Letters, 2009, 42, 505.

31. Zhongqiu Liu,  Shengxin Lu, Cuihua Zhao,  Zhaozhen Cao,  Yanshuai Wang,  Yulan Zhang, Chengbiao Zhao, Wei Liu, Jinhua Zhan, Jinting Liu, and Rimo Xi1*. Preparation of anti-Danofloxacin Antibody and Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Danofloxacin Residue in Chicken Liver. J. of the Science of Food and Agriculture, 2009, 89, 1115..

32. Zhao, C.; Liu, W.; Ling, H.; Lu, S.; Zhang, Y.; Liu, J.; Xi, R*. Preparation of anti-Gatifloxacin Antibody and Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Gatifloxacin Residue in Milk. J. Agric. Food Chem. 2007, 55, 6879.

33. Liu, W.; Zhao, C.; Zhang, Y.; Lu, S.; Liu, J.; Xi, R*. Preparation of Polyclonal Antibodies to a Derivative of 1-Aminohydantoin (AHD) and Development of an Indirect Competitive ELISA for Detection of Nitrofurantoin Residue in Water. J. Agric. Food Chem. 2007, 55, 6829.

34. Xu, P.; Qiu,  J.; Zhang, Y.; Chen,  J.; Wang, G. P.; Yan, B.; Song, J.; Xi, R.; Deng, Z.; Ma, C. Efficient whole-cell biocatalytic synthesis of N-acetyl-D-neuraminic acid, Adv. Synth. Catal. 2007, 349, 1614.

35. Zhang, Y. L.; Lu, S. X.; Liu, W.; Zhao, C. B.; Xi, R*. Preparation of anti-Tetracycline Antibodies and Development of an Indirect Heterologous cELISA Assay to Detect Residue of Tetracycline in Milk. J. Agric. Food Chem. 2007, 55, 211.

36. Lu, S. X.; Zhang, Y. L.; Liu, J. T.; Zhao, C. B.; Liu, W.; Xi, R*. Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver. J. Agric. Food Chem. 2006, 54, 6995.

37. Zhang, X.; Xi, R.; Liu, J.; Jiang, J.; Wang, G.; Zeng, Q. Molecular and electronic structures as well as vibrational spectra assignment of biphenyl, 2,20- and 4,40-dichlorobiphenyl from density functional calculations, J. Mol. Struc-Theochem. 2006, 763, 67.

38. Xi R, Wang B, Abe M, Ozawa Y, Kinoshita I, and Isobe K. Tetranuclear Mo2Rh2 Complexes Obtained from Reactions between Triple Cubane-Type Oxide Cluster [(RhCp*)4Mo4O16] (Cp* =η5-C5Me5) and Methanethiol: [{Cp*Rh(μ-SMe)3MoO2}2(μ-O)] and [{Cp*Rh(μ-SMe)3 MoO}2 (μ-X)(μ-Y)] (X, Y = O and X=O, Y=S).  Synthesis, X-ray Crystal Structures and Dynamic Behavior in Non-Aqueous Media, Bulletin of the Chemical Society of Japan, 1999, 72, 1985.

39. Sita LR, Xi R, Yap GPA, Liable-Sands LM, and Rheingold AL. High Yield Synthesis and characterization of Sn6(μ3-O)4(μ3-OSiMe3)4: A Novel Main Group Cluster for the Support of Multiple Transition Metal Centers, J. Am. Chem. Soc., 1997, 119, 756.

40. Sita LR, Babcock JR, and Xi R. Facile Metathetical Exchange Between Carbon Dioxide and the Divalent Group 14 Bisamides, M[N(SiMe3)2]2 (M = Ge and Sn), J. Am. Chem. Soc., 1996, 118, 10912.

41. Xi R, Babcock JR, and Sita LR. A. Thermal Reductive Elimination Route to Perbutylated Cyclopolystannanes, Organometallics, 1996, 15, 2849.

42. Xi R, Abe M, Suzuki T, Nishioka T, and Isobe K. Synthesis and Characterization of Pentamethylcyclopentadienylrhodium (III) and –iridium(III) Complexes with 1,2-Benzenedi- thiolate: [(Cp*Rh)2(μ(S)-1,2-C6H4S2-S,S’)2], [(Cp*Rh)2(μ(S)-1,2-C6H4S2-S,S’) (μ(S)-1,2- C6H4S(SO)-S,S’) 2] and [(Cp*Ir)(1,2-C6H4S2-S,S’)] (Cp*=η5-C5Me5), J. Organomet. Chem., 1997, 549, 117.

43. Xi R and Sita LR. Mechanistic Details for Metathetical Exchange between XCO (X = O and RN) and the Tin(II) Dimer,{Sn[N(SiMe3)2](μ-OBut)}2, Inorg. Chim. Acta, 1997, 270, 118.

44. Xi R, Wang B, Isobe K, Nishioka T, Toriumi K, and Ozawa Y.  Isolation and X-ray Crystal Structure of a New Octamolybdate: [(RhCp*)2(μ-SCH3)3]4[Mo8O26] 2CH3CN (Cp*=η5-C5Me5), Inorg. Chem., 1994, 33, 833.

45. Xi R, Wang B, Abe M, Ozawa Y, and Isobe K. Fragmentation of Triple Cubane- Framework in [(RhCp*)4Mo4O16] (Cp*=η5-C5Me5) by MeSH into Tetranuclear Parts in [{Cp*Rh(μ-SMe)3MoO}2(μ-O)2] and [{Cp*Rh(μ-SMe)3MoO}2(μ-O)(μ-S)] Providing a Novel System for Studying Stereodynamics of Thiolate Complexes Chem. Lett., 1994, 1177.

46. Xi R, Wang B, Abe M, Ozawa Y, and Isobe K.  New Linear-Type Tetranuclear Complexes, [{Cp*Rh(μ-SMe)3MoO2}2(μ-O)] (Cp*=η5-C5Me5), Chem. Lett., 1994, 323.